The investigation of hemoglobin in hypertension; spectroscopic and thermodynamic analysis
H.Filsoofiana,b, S. Akbarib, A. Akbaria*
a GreenNanoTech Kft, Király Utca 80, Budapest, 1068, Hungary
b NanoSciTec GmbH, Hermann Weinhauser str. 67, Munich, 81867, Germany
Abstract
Hypertension is a disease with a significant risk factor for heart failure and stroke. One of the main reasons behind heart failure and stroke in hypertensive patients is the reduction of erythrocytes deformability. An influential factor in determining erythrocyte deformability is the erythrocyte’s cytoplasmic viscosity. The majority of erythrocyte cytoplasm is composed of hemoglobin. This report investigates structural and thermodynamical hemoglobin changes in induced-hypertensive rats using circular dichroism (CD) spectroscopy and differential scanning calorimetry (DSC) techniques. The obtained results from hypertensive cases reveal increased flexibility and a reduction of compactness in hemoglobin structures. Additionally, the availability of heme groups and hydrophobic residues in hemoglobin structures decreased. This process led to a lack of oxygen binding to heme groups. Furthermore, hemoglobin aggregation occurs and causes an increase in cytoplasmic viscosity and a decrease in erythrocyte deformability.
Keywords: Hypertension, Hemoglobin, Erythrocyte’s deformability
© Article info: Accepted by: 2 May 2022, Published by: 17 may 2022.
Doi number: https://doi.org/10.52319/j.nanoscitec.2022.10
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